Little is known about the prevalence of immunodeficiency virus (HIV) and hepatitis C virus (HCV) co-infection in the North region of Brazil. This study aimed to describe the seroprevalence of HCV infection in newly-diagnosed patients with HIV in Belém, Pará. A cross-sectional study was conducted between May 2016 and June 2017, which enrolled 185 individuals. Data collection involved the use of a questionnaire and the collection of blood sample for anti-HCV screening. Results indicated only a single individual who was seropositive for both HIV/HCV infection, which is considered a very low prevalence (0.5%) when compared to other Brazilian regions.