Objectives: To validate the quantitative analysis of phospholipid in liposomal amphotericin B for lyophilized injection by UV-Vis spectrophotometer. Materials and methods: Liposomal amphotericin B for lyophilized injection produced by Department of Pharmaceutics, Hanoi University of Pharmacy is analyzed with Hitachi model U-1800 spectrophotometer by measuring the amount of phosphor in phospholipid