Antibiotics are the first line of defence against bacterial infections. Overuse/misuse of antibiotics has led to the emergence of antibiotics resistant bacterial strains, making them ineffective. Alternative approaches should be sought to handle this challenge. Bile salt is a cation compounded form of bile acid and is synthesized in the liver. Reduction in the flow of bile salts has led to bacterial translocation in the body. Therefore, the aim of the present study was to evaluate the in vitro effect of bile salt on E. coli and S. aureus.