Blood stream infections are very common in the paediatric age group and these are one of the common causes of morbidity and mortality in neonates and children. Objectives of the study are to isolate the different organisms from Blood cultures of Neonates by BACTEC method and know their Antibiotic susceptibility patterns. We processed 100 Blood samples of the Neonates from PICU of NIMS Medical College and Hospital between January and June 2016 (06 months).