Human bocavirus (HBoV) was first identified as parvovirus in 2005 in nasopharyngeal aspirates of children with lower respiratory tract infection. The objectives of this study were to determine HBoV as a cause of acute lower respiratory tract infection(ALRTI) in children by investigating the presence of HBoV DNA, to evaluate diagnostic efficiency of HBov IgM assay in the serum of the studied patients and to assess the impact of the viral load on the clinical manifestations of children with positive HBoV DNA samples. We investigated 123-nasopharyngeal aspirate samples of hospitalized children with ALRTI by Real-time PCR for detection of HBoV DNA and estimating its viral load. Estimation of immunoglobulin M (IgM) by ELISA in patient’s serum samples was another method used for diagnosis of HBoV infection. There were 19 patients with positive nasopharyngeal aspirate samples by PCR among them there were 16 serum samples positive for HBoV IgM by ELISA (sensitivity was 84.2%). Longer hospital stay and severe ALRTI signs were detected in child