Bacterial vaginosis is caused by an imbalance of the organisms that naturally exist in the vagina. The importance of bacterial vaginosis with respect to pregnant women’s health is emphasized by the association between bacterial vaginosis and adverse outcome of pregnancy. The aim of present study was to evaluate the direct smear microscopy and culture for determination of bacteria from vaginal discharge of pregnant women. Vaginal discharge in women is sometimes caused by candida and aerobic bacteria organisms like E.coli, staphylococcus aureus, and β- haemolytic streptococcus.